Background: There is now a worldwide epidemic of cardiovascular disease, atherosclerosis being the most common. Mostly the atherosclerotic-related diseases are acquired diseases. Tobacco is the most critical risk factor for atherosclerotic diseases which can be controlled and preventable. In this context, the aim of this study was to evaluate the consequences of chronic use of tobacco on the lipid profile of the middle-aged population. Aim: Aim of the study was to evaluate the impact of various tobacco habits on the serum lipid profile. Materials and methods: Patients who attended the outpatient department (OPD) of Oral Medicine Diagnosis and Radiology (OMDR), screened randomly for habits of tobacco smoking and chewing, 45 patients were selected who had a habit of smoking and/or chewing tobacco, as a study group and 15 patients with no habits were chosen as the control group. Detailed information was taken regarding the frequency, duration, content of the form of tobacco they used. The patients were subjected to estimation of lipid profile. The values were recorded and statistically analyzed. Comparison of lipid profile and atherosclerotic index between tobacco smokers, tobacco chewers and nonsmokers/chewers were done using appropriate statistical analysis test. A p-value < 0.05 was considered to be statistically significant. Results: There was a significant increase in the total cholesterol (TC), low-density lipoprotein (LDL) and atherosclerotic index (AI) levels were observed in the tobacco users when we compared with nontobacco users. However, high-density lipoproteins (HDL) which is good cholesterol has a high level in nonusers compared to tobacco users Conclusion: Increased levels of TC and LDL are the risk factors in the developing coronary heart disease. As tobacco is the known risk factor for atherosclerosis, extensive awareness is essential and can save the lives of many.