Evaluation of the Efficacy of a Single Subgingival Irrigation with Ozonated Water on Gingival Inflammation, Lactate Dehydrogenase Enzyme, and Alkaline Phosphatase Enzyme Activity in GCF in the Subjects undergoing Fixed Orthodontic Appliance Therapy: A Clinical and Biochemical Study
Citation Information :
Padhye AM, Tambade NC. Evaluation of the Efficacy of a Single Subgingival Irrigation with Ozonated Water on Gingival Inflammation, Lactate Dehydrogenase Enzyme, and Alkaline Phosphatase Enzyme Activity in GCF in the Subjects undergoing Fixed Orthodontic Appliance Therapy: A Clinical and Biochemical Study. J Contemp Dent 2018; 8 (1):33-38.
The aqueous form of ozone is a potential antiseptic and anti-inflammatory agent, shows less cytotoxicity than gaseous ozone or established antimicrobials, and thus fulfills optimal cell biological characteristics in terms of biocompatibility for oral application.
To evaluate the efficacy of a single subgingival irrigation with ozonated water on gingival inflammation and lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activity in the gingival crevicular fluid (GCF) in patients undergoing fixed orthodontic therapy.
Materials and methods
A total of 15 participants having full mouth fixed orthodontic appliances for at least past 3 months were enrolled in the study. The study was conducted for 28 days during which GCF was collected for analysis of LDH and ALP activity; plaque index (PI), gingival index (GI), gingival bleeding index (GBI), and probing pocket depth (PPD) were recorded and scaling and root planing (SRP) was performed at baseline,14, 21, and 28 days. Subgingival irrigation with aqueous ozone was performed after SRP at baseline. Wilcoxon signed rank test and Spearman's ranked correlation and nonparametric methods were used for statistical analysis. For all the tests, a p-value of 0.05 or less was considered for statistical significance.
Subgingival ozone irrigation is an effective method that can be performed during monthly visits on orthodontic patients to reduce the gingival inflammation which is caused due to the plaque-retentive nature of orthodontic appliances.
How to cite this article
Tambade NC, Padhye AM. Evaluation of the Efficacy of a Single Subgingival Irrigation with Ozonated Water on Gingival Inflammation, Lactate Dehydrogenase Enzyme, and Alkaline Phosphatase Enzyme Activity in GCF in the Subjects undergoing Fixed Orthodontic Appliance Therapy: A Clinical and Biochemical Study. J Contemp Dent 2018;8(1):33-38.
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